Journal article
P. Kozielewicz, Rawan Shekhani, Stefanie Moser, Carl-Fredrik Bowin, Janine Wesslowski, G. Davidson, G. Schulte
ACS Pharmacology & Translational Science, 2021
ACS Pharmacology & Translational Science, 2021
Semantic Scholar
DOI
PubMedCentral
PubMed
Cite
Cite
APA
Click to copy
Kozielewicz, P., Shekhani, R., Moser, S., Bowin, C.-F., Wesslowski, J., Davidson, G., & Schulte, G. (2021). Quantitative Profiling of WNT-3A Binding to All Human Frizzled Paralogues in HEK293 Cells by NanoBiT/BRET Assessments. ACS Pharmacology &Amp; Translational Science.
Chicago/Turabian
Click to copy
Kozielewicz, P., Rawan Shekhani, Stefanie Moser, Carl-Fredrik Bowin, Janine Wesslowski, G. Davidson, and G. Schulte. “Quantitative Profiling of WNT-3A Binding to All Human Frizzled Paralogues in HEK293 Cells by NanoBiT/BRET Assessments.” ACS Pharmacology & Translational Science (2021).
MLA
Click to copy
Kozielewicz, P., et al. “Quantitative Profiling of WNT-3A Binding to All Human Frizzled Paralogues in HEK293 Cells by NanoBiT/BRET Assessments.” ACS Pharmacology &Amp; Translational Science, 2021.
BibTeX Click to copy
@article{p2021a,
title = {Quantitative Profiling of WNT-3A Binding to All Human Frizzled Paralogues in HEK293 Cells by NanoBiT/BRET Assessments},
year = {2021},
journal = {ACS Pharmacology & Translational Science},
author = {Kozielewicz, P. and Shekhani, Rawan and Moser, Stefanie and Bowin, Carl-Fredrik and Wesslowski, Janine and Davidson, G. and Schulte, G.}
}
Abstract
The WNT signaling system governs critical processes during embryonic development and tissue homeostasis, and its dysfunction can lead to cancer. Details concerning selectivity and differences in relative binding affinities of 19 mammalian WNTs to the cysteine-rich domain (CRD) of their receptors—the ten mammalian Frizzleds (FZDs)—remain unclear. Here, we used eGFP-tagged mouse WNT-3A for a systematic analysis of WNT interaction with every human FZD paralogue in HEK293A cells. Employing HiBiT-tagged full-length FZDs, we studied eGFP-WNT-3A binding kinetics, saturation binding, and competition binding with commercially available WNTs in live HEK293A cells using a NanoBiT/BRET-based assay. Further, we generated receptor chimeras to dissect the contribution of the transmembrane core to WNT-CRD binding. Our data pinpoint distinct WNT-FZD selectivity and shed light on the complex WNT-FZD binding mechanism. The methodological development described herein reveals yet unappreciated details of the complexity of WNT signaling and WNT-FZD interactions, providing further details with respect to WNT-FZD selectivity.
