Journal article
Bożena Kuźniewska, D. Cysewski, Michał Wasilewski, Paulina Sakowska, J. Miłek, Tomasz M. Kuliński, P. Kozielewicz, M. Dadlez, A. Chacińska, A. Dziembowski, M. Dziembowska
bioRxiv, 2019
bioRxiv, 2019
Semantic Scholar
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APA
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Kuźniewska, B., Cysewski, D., Wasilewski, M., Sakowska, P., Miłek, J., Kuliński, T. M., … Dziembowska, M. (2019). Mitochondria biogenesis in the synapse is supported by local translation. BioRxiv.
Chicago/Turabian
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Kuźniewska, Bożena, D. Cysewski, Michał Wasilewski, Paulina Sakowska, J. Miłek, Tomasz M. Kuliński, P. Kozielewicz, et al. “Mitochondria Biogenesis in the Synapse Is Supported by Local Translation.” bioRxiv (2019).
MLA
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Kuźniewska, Bożena, et al. “Mitochondria Biogenesis in the Synapse Is Supported by Local Translation.” BioRxiv, 2019.
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@article{bo2019a,
title = {Mitochondria biogenesis in the synapse is supported by local translation},
year = {2019},
journal = {bioRxiv},
author = {Kuźniewska, Bożena and Cysewski, D. and Wasilewski, Michał and Sakowska, Paulina and Miłek, J. and Kuliński, Tomasz M. and Kozielewicz, P. and Dadlez, M. and Chacińska, A. and Dziembowski, A. and Dziembowska, M.}
}
Abstract
Synapses are the regions of the neuron that enable the transmission and propagation of action potentials on the cost of high energy consumption and elevated demand for mitochondrial ATP production. The rapid changes in local energetic requirements at dendritic spines imply the role of mitochondria in the maintenance of their homeostasis. Using global proteomic analysis supported with complementary experimental approaches, we show that an important pool of mitochondrial proteins is locally produced at the synapse indicating that mitochondrial biogenesis takes place locally to maintain the pool of functional mitochondria in axons and dendrites. Furthermore, we show that stimulation of synaptoneurosomes induces the local synthesis of mitochondrial proteins that are transported to the mitochondria and incorporated into the protein supercomplexes of the respiratory chain. Synopsis Mitochondria biogenesis in the synapse is supported by local translation Mitochondrial proteins represent a large pool of proteins synthesized locally at the synapse Newly synthesized mitochondrial proteins are imported into the mitochondria and incorporated into the respiratory chain complexes Uncoupling of mitochondria and blocking mitochondrial import inhibits incorporation of de novo synthesized proteins into the mitochondrial protein complexes
